Plasmid_Backbone
pIJ6021

Part:BBa_K4590011:Design

Designed by: Caue Augusto Boneto Goncalves   Group: iGEM23_USP-Brazil   (2023-10-02)


Plasmid backbone of malonate production


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 43
    Illegal XbaI site found at 16
    Illegal PstI site found at 4
    Illegal PstI site found at 4847
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 43
    Illegal PstI site found at 4
    Illegal PstI site found at 4847
    Illegal NotI site found at 6727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 43
    Illegal BglII site found at 52
    Illegal BglII site found at 2284
    Illegal BglII site found at 2755
    Illegal BglII site found at 3573
    Illegal BamHI site found at 22
    Illegal XhoI site found at 5644
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 43
    Illegal XbaI site found at 16
    Illegal PstI site found at 4
    Illegal PstI site found at 4847
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 43
    Illegal XbaI site found at 16
    Illegal PstI site found at 4
    Illegal PstI site found at 4847
    Illegal NgoMIV site found at 572
    Illegal NgoMIV site found at 698
    Illegal NgoMIV site found at 708
    Illegal NgoMIV site found at 1373
    Illegal NgoMIV site found at 2428
    Illegal NgoMIV site found at 3060
    Illegal NgoMIV site found at 5219
    Illegal AgeI site found at 1257
    Illegal AgeI site found at 2527
    Illegal AgeI site found at 2867
    Illegal AgeI site found at 4537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6067
    Illegal BsaI site found at 6342
    Illegal BsaI site found at 7524
    Illegal SapI site found at 600
    Illegal SapI.rc site found at 895


Design Notes

This sequence is all plasmid without the interested gene, on the final of plasmid sequence you can put the sequence of your interest gene


Source

NCBI:txid173259

References